![]() ![]() This observation may be of interest not only in relation to tissue subjected to this We found a nonlinear shrinking of 13☑7% in the x- and y-directions and a spatial shrinking up to 50% in some regions after theĭehydration and staining process. Made it possible to study and measure aspects of the complexity of dehydration and staining artifacts. The silver-enhanced vessels acted as natural markers and ![]() Hippocampal region re¯ecting known subregional structures. We found a high variation of capillary density in the Stereograms and `shadow' 3-D images were produced. Images were frame grabbed during optical slicing, and classic Interfere with further dehydration and counterstaining. The developed gold staining is stable and does not In the vessels became encased in silver and thereby made visible. In this way, the colloidal gold particles The brains were post-®xed with glutaraldehyde and vibratomized intoġ00-ìm-thick slices, and the gold particles were developed by autometallography. Glutaraldehyde and a 408C gold±gelatine solution. In order to visualize the vascular system of the rat brain, 10 Wistar rats were perfused transcardially with Received and in revised form 4 August 1997 Silver enhancement of colloidal gold particlesĪ R N E A N D R E A S E N and G ORM DAN SCH ERĭepartment of Neurobiology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, Denmark Optical slicing and 3-D characterization of hippocampalĬapillaries in the rat visualized by autometallographic ![]()
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